【目的】 为获得刀豆转录组信息特征，丰富其基因数据库，并为后续遗传图谱构建、品种选育以及解析刀豆黄酮类化 合物次生代谢途径和调控机制研究提供基础数据。【方法】 基于Illumina 6000平台对刀豆各组织部位进行转录组测序，组装所 有转录本并进行去冗余。对获得的去冗余基因（unigenes）在NR、Swiss-Prot、KEGG、KOG、GO五大数据库中进行功能注释。 随后进行基因表达量分析，并对刀豆各组织部位及3个不同时期的果荚进行基因差异表达分析。对差异表达基因进行京都基 因与基因组百科全书（KEGG）富集分析，并对类黄酮生物合成途径相关基因进行注释和分类。【结果】（1）测序组装后共获得 137 278条去冗余的 unigenes。（2）在五大数据库中总计获得功能注释 76 793条（占 unigenes总量的 55.95%），具体为：KOG 数 据库 40 965条（占注释总量的 29.84%）、KEGG数据库 32 776条（占注释总量的 23.88%）、NR数据库 67 373条（占注释总量的 49.08%）、Swiss-Prot 数据库 56 397 条（占注释总量的 41.08%）、GO 数据库 31 399 条（占注释总量的 22.87%）。（3）通过比较 3 个不同时期的刀豆果荚，分别鉴定到1 015、6 445和7 207条差异表达基因。（4）KEGG富集分析表明，差异表达基因显著富 集于植物激素信号转导、苯丙烷代谢、类黄酮生物合成等通路。（5）在类黄酮生物合成途径中共注释到57个基因，这些基因 可能在刀豆黄酮类化合物生物合成中发挥重要作用。【结论】 成功获取刀豆的转录组数据，组装获得大量unigenes并进行功能 注释，揭示不同组织部位及果荚发育时期的基因表达差异及其富集通路，特别是发现57个与类黄酮生物合成相关的潜在关 键基因。这些结果显著丰富刀豆基因数据库，为刀豆的遗传图谱构建、分子育种以及深入解析其黄酮类化合物次生代谢途 径和调控机制奠定数据基础。

Objective To obtain transcriptome information characteristics of Canavalia gladiata（sword bean）， enrich its gene database， and provide fundamental data for subsequent genetic map construction， variety breeding，and research on the secondary metabolic pathways and regulatory mechanisms of flavonoid compounds in sword bean. Methods Transcriptome sequencing of various tissue parts of sword bean was performed using the Illumina 6000 platform. All transcripts were assembled and deduplicated. The obtained deduplicated genes （unigenes） were functionally annotated in five major databases including NR，Swiss-Prot，KEGG，KOG，GO. Gene expression analysis was conducted，followed by differential gene expression analysis of various tissue parts and pods at three different developmental stages. Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis was performed on differentially expressed genes （DEGs），and genes related to the flavonoid biosynthesis pathway were annotated and classified. Results（1）A total of 137 278 deduplicated unigenes were obtained after sequencing and assembly.（2） Functional annotations were obtained for 76 793 unigenes （55.95% of total unigenes） across the five databases ： KOG database （40 965 annotations，29.84% of total annotated），KEGG database （32 776 annotations， 23.88%）， NR database （67 373 annotations， 49.08%）， SwissProt database （56 397 annotations，41.08%），and GO database （31 399 annotations，22.87%）.（3） Comparison of sword bean pods at three different developmental stages identified 1 015，6 445，and 7 207 DEGs，respectively.（4） KEGG enrichment analysis revealed that DEGs were significantly enriched in pathways such as plant hormone signal transduction，phenylpropanoid metabolism，and flavonoid biosynthesis.（5） Fifty-seven genes were annotated in the flavonoid biosynthesis pathway，which may play important roles in the biosynthesis of flavonoid compounds in sword bean. Conclusion The transcriptome data of sword bean were successfully obtained，and a large number of unigenes were assembled and functionally annotated. The study revealed gene expression differences and enriched pathways across different tissue parts and pod developmental stages，particularly identifying 57 potential key genes related to flavonoid biosynthesis. These results enrich the sword bean gene database and provide important data foundations for genetic map construction，molecular breeding，and in-depth analysis of the secondary metabolic pathways and regulatory mechanisms of flavonoid compounds in sword bean.

R282.5

国家自然科学基金资助项目（编号：32170291）