【目的】 明确最佳提取高良姜中山奈酚的工艺及观察其抗氧化活性。【方法】 基于单因素试验的数据，采用 BoxBehnken中心组合试验设计和响应面（RSM）分析法，结合高效液相色谱（HPLC）法，以山奈酚含量为响应值绘制响应面图和 等高线图，优选乙醇浓度、料液比、提取时间和提取温度的最佳参数。通过二苯代苦味酰肼（DPPH）自由基清除实验对山奈 酚抗氧化活性进行评估。【结果】 最佳工艺条件为乙醇浓度84%，料液比1∶80，提取时间2 h，提取温度82 ℃，在此工艺条件 下，从高良姜中提取出的山奈酚含量可达0.034 16%。DPPH自由基清除实验中，高良姜提取物浓度在1 mg/mL时清除率达到 91.20%。【结论】 利用Box-Behnken响应面设计法得到了高良姜中山奈酚优化提取工艺，该工艺方便可行。通过DPPH自由基 清除实验，证明了高良姜中山奈酚具有抗氧化活性。

Objective To determine the optimal extraction process for kaempferol from Alpinia officinarum Hance and to investigate its antioxidant activity. Methods Based on single-factor experimental data，this study employed the Box-Behnken central composite design and response surface methodology（RSM）， combined with highperformance liquid chromatography（HPLC），to plot response surface and contour maps using kaempferol content as the response value. The optimal parameters for ethanol concentration，solid-liquid ratio，extraction time，and extraction temperature were selected. The antioxidant activity of kaempferol was comprehensively evaluated by calculating the radical scavenging rate using the DPPH radical scavenging assays. Results The optimal extraction conditions were determined to be an ethanol concentration of 84%，a solid-liquid ratio of 1∶80，an extraction time of 2 hours，and an extraction temperature of 82 ℃. Under these conditions，the kaempferol content extracted from Alpinia officinarum Hance reached 0.034 16%. In the DPPH radical scavenging assay，the extract showed a scavenging rate of 91.20% at a concentration of 1 mg/mL. Conclusion The optimized extraction process for kaempferol from Alpinia officinarum Hance was obtained using the Box-Behnken response surface design method，and this process is practical and feasible. The DPPH radical scavenging assay demonstrated that kaempferol from Alpinia officinarum Hance possesses antioxidant activity.

R282.5

广东省重点学科科研项目（编号：2019-GDXK-0025，2021ZDJS035）；湛江市科技计划项目（编号：2022A01111）