【目的】 观察健阳片对肝郁肾虚型勃起功能障碍大鼠的治疗作用及机制。【方法】 将60只雄性SD大鼠随机分为正常组、 模型组、他达拉非组和健阳片低、中、高剂量组。除正常组，其他各组大鼠采用氢化可的松肌肉注射联合束缚四肢复合造 模方法连续14 d诱发肝郁肾虚型阳痿模型。成功造模后，连续给药28 d。记录与对比实验前后大鼠体质量差，进行大鼠旷场 试验、爬背试验，酶联免疫吸附分析（ELISA）检测大鼠阴茎海绵体中一氧化氮合成酶（NOS）、环磷酸腺苷（cAMP）、环磷酸 鸟苷（cGMP）水平，Griess试剂比色法检测大鼠阴茎海绵体中一氧化氮（NO）水平。【结果】 与正常组比较，模型组大鼠旷场试 验水平活动格数减少，爬背试验扑捉潜伏期延长、扑捉次数减少，体质量降低，阴茎海绵体组织NOS、NO、cGMP、cAMP 水平降低，差异均有统计学意义（P＜0.05或 P＜0.01）；与模型组比较，他达拉非组和健阳片中、高剂量组大鼠旷场试验水 平活动格数、垂直活动次数增加，爬背试验扑捉潜伏期缩短、扑捉次数增加，体质量升高，阴茎海绵体组织 NOS、NO、 cGMP、cAMP水平升高，差异均有统计学意义（P＜0.05或 P＜0.01）；各干预组间上述指标比较，差异均无统计学意义（P＞ 0.05）。【结论】 健阳片可有效改善大鼠肝郁肾虚型勃起功能障碍，其机制可能与调节NO-cGMP信号通路有关。

Objective To investigate the therapeutic effects and mechanisms of Jianyang Tablets（mainly with the functions of tonifying the kidneys，replenishing essence，invigorating yang，and alleviating erectile dysfunction） on erectile dysfunction（ED） in rats with liver depression and kidney deficiency syndrome. Methods The 60 male SD rats were randomly divided into a normal group，a model group，a Tadalafil group，and low-，medium-， and high- dose Jianyang Tablets groups. Except for the normal group， all other groups underwent a 14-day composite modeling protocol combining intramuscular hydrocortisone injections and limb immobilization to induce ED with liver depression and kidney deficiency syndrome. Treatments were administered for 28 consecutive days after successful modeling. The body mass differences were recorded and compared before and after the experiment.Behavioral assessments included open-field test and mounting test were conducted. Enzyme-linked immunosorbent assay（ELISA） was used to measure nitric oxide synthase（NOS），cyclic adenosine monophosphate（cAMP），and cyclic guanosine monophosphate（cGMP） levels in penile cavernous tissues. Nitric oxide（NO） level was quantified using the Griess reagent colorimetric method. Results Compared with the normal group，the model group exhibited reduced horizontal activity grid counts in the open-field test，prolonged mounting latency，decreased mounting frequency， decreased body mass， and reduced levels of NOS， NO， cGMP， and cAMP in penile cavernous tissues，with statistical significance（P＜0.05 or P＜0.01）. Compared with the model group，the Tadalafil group and medium- and high-dose Jianyang Tablets groups showed increased horizontal activity grid counts and vertical activity counts in the open-field test，shortened mounting latency，elevated mounting frequency，increased body mass， and upregulated levels of NOS， NO， cGMP， and cAMP in penile cavernous tissues， with statistical significance（P＜0.05 or P＜0.01）. No significant differences were observed among the intervention groups for the aforementioned indices（P＞0.05）. Conclusion Jianyang Tablets effectively improves erectile function in rats with liver depression and kidney deficiency syndrome，potentially mediated by modulation of the NO-cGMP signaling pathway.

R285.5

广东省中医药局科研项目（编号：20231159）；广州中医药大学“双一流”与高水平大学学科后备人才培育项目（编号：A1-2606- 22-415-016）