【目的】 使用高通量测序技术研究三角草的转录表达情况。【方法】 通过提取三角草的总RNA，进行文库构建、测序以 及de novo组装，得到单基因簇（unigene）序列，然后进行注释和比对，进而获得三角草转录组的遗传信息。【结果】 共识别出 29 325条unigene，平均长度为1 026 bp，N50为1 697 bp。其中，20 338条unigene在至少一个数据库中获得了功能注释，三 角草与芦笋（Asparagus officinalis）的序列最为相似。在京都基因与基因组百科全书（KEGG）数据库中，13 392条unigene得到了 注释，包括黄酮合成通路和泛醌及其他萜烯类醌的生物合成等通路基因，鉴定出三角草皂苷合成的上游基因35个。在基因 本体论（GO）数据库中，16 728条三角草unigene获得了注释，涵盖了细胞解剖实体、结合和生物过程等方面。通过微卫星鉴 定工具（MISA）对组装后的 11 486 条长度超过 1 000 bp 的 unigene 进行了简单序列重复（SSR）检测，共鉴定出 5 178 个 SSR 位 点，并使用Primer 3.0进行引物设计。通过将三角草unigene与转录因子数据库比对，鉴定出bHLH、MYB和WRKY等转录因子 657个。【结论】 转录组数据为三角草的药用活性成分合成通路及功能基因的研究提供了基础，也有助于其资源的保护和开发。

Objective To study the transcriptional expression of Chlorophyti Laxi R. Br. using high-throughput sequencing technology. Methods Total RNA of Chlorophtum laxum R. Br. was extracted， followed by library construction， sequencing， and de novo assembly to obtain unigene sequences. These sequences were then annotated and compared to acquire genetic information of the Chlorophtum laxum R. Br. transcriptome. Results A total of 29 325 unigene were identified， with an average length of 1 026 bp and an N50 of 1 697 bp. Among them， 20 338 unigene were functionally annotated in at least one database， with Chlorophtum laxum R. Br. showing the highest sequence similarity to Asparagus officinalis. In the Kyoto Encyclopedia of Genes and Genomes （KEGG） database， 13 392 unigene were annotated， including genes involved in flavonoid biosynthesis and ubiquinone and other terpenoid-quinone biosynthesis pathways. Thirty-five upstream genes related to Chlorophtum laxum R. Br. saponin biosynthesis were identified. In the Gene Ontology （GO） database，16 728 unigene were annotated， covering cellular anatomical entities， binding， and biological processes. Using the Microsatellite Identification Tool（MISA），11 486 assembled unigene longer than 1 000 bp were analyzed for simple sequence repeats （SSRs），resulting in the identification of 5 178 SSR loci，for which primers were designed using Primer 3.0. By comparing Chlorophtum laxum R. Br. unigene with the transcription factor database， 657 transcription factors， including bHLH， MYB， and WRKY， were identified. Conclusion The transcriptome data provide a foundation for studying the biosynthetic pathways and functional genes of medicinal active components in Chlorophtum laxum R. Br.，and also contribute to the conservation and development of its resources.

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广东省农作物种质资源普查与收集项目（编号：2023N13H00001）；广东省特色经济作物与南药资源圃运行维护项目（编号： 2023NBH00015）；广东省农业科学院乡村振兴地方分院和专家工作站工作经费项目（编号：2024工作站07）