【目的】 建立基于功效主治的外感发热药浴方（由连翘、前胡、荆芥穗、板蓝根、石膏、菊花、淡竹叶等药味构成）质 量标准。【方法】 薄层色谱鉴别（TLC）法建立君药前胡、连翘的鉴别方法。酶联免疫吸附分析（ELISA）测定方中主要成分的白 细胞介素 1β（IL-1β）调控活性。高效液相色谱（HPLC）法测定 10批样品中活性成分的含量。【结果】 供试品中成功检出前胡、 连翘斑点。ELISA法发现方中白花前胡甲素、胡薄荷酮、芦丁、白花前胡乙素、连翘苷、（R，S）-告依春等活性成分。10批 样品含量测定结果显示，白花前胡甲素含量为0.493 ~ 0.694 mg·mL-1 。【结论】 基于功效主治建立了外感发热药浴方的TLC鉴 别和HPLC含量测定方法，所得标准能够更加准确地对药效进行控制。

Objective To establish a quality standard for the medicinal bath formula for treating exogenous fever （composed of Forsythiae Fructus，Peucedani Radix，Schizonepetae Spica，Isatidis Radix，Gypsum Fibrosum， Chrysanthemi Flos， Lophatheri Herba， etc.） based on its efficacy and indications. Methods Thin-layer chromatography （TLC） was used to establish identification method for the monarch drugs Forsythiae Fructus and Peucedani Radix. Enzyme-linked immunosorbent assay（ELISA） was employed to measure the interleukin 1β （IL-1β） regulatory activity of the main components in the formula. High-performance liquid chromatography （HPLC） was used to determine the content of active components in 10 batches of samples. Results Spots of Forsythiae Fructus and Peucedani Radix were successfully detected in the test samples. ELISA identified active components in the formula，including praeruptorin A，pulegone，rutin，praeruptorin B，forsythoside，and （R， S）-goitrin. The content determination results of 10 batches of samples showed that the content of praeruptorin A ranged from 0.493 to 0.694 mg·mL-1 . Conclusion Based on its efficacy and indications，TLC identification and HPLC content determination methods were established for the medicinal bath formula for treating exogenous fever. The obtained standard can more accurately control the efficacy of the formula.

R282.5

广东省重点领域研发计划项目（编号： 2020B020221002）；佛山市自筹经费类科技计划项目（编号：2220001005592）