【目的】 观察大黄素对脂多糖（LPS）诱导的足细胞损伤的改善作用及机制。【方法】 以人肾小球足细胞为研究对象，将其 随机分为空白组，LPS组，大黄素低、中、高剂量组，溶血磷脂酸[LPA，RhoA/Rho蛋白相关卷曲螺旋激酶（ROCK）激活剂] 组。细胞计数试剂盒8（CCK-8）法和5-乙炔基-2'-脱氧尿苷（EdU）染色法测定足细胞增殖能力，Transwell实验检测足细胞迁 移能力，流式细胞仪检测足细胞凋亡，酶联免疫吸附分析（ELISA）检测足细胞上清液中炎症因子肿瘤坏死因子 α（TNF-α）、 白细胞介素（IL）-1β、IL-18水平，Western Blot法检测足细胞中RhoA、ROCK蛋白表达水平。【结果】 LPS组足细胞的光密度 （OD）450 nm、EdU 阳性细胞率、迁移数低于空白组，细胞凋亡率，上清液中 TNF-α、IL-1β、IL-18 水平，细胞中 RhoA、 ROCK 蛋白表达水平高于空白组，差异均有统计学意义（P < 0.05）；与 LPS 组比较，大黄素低、中、高剂量组足细胞的 OD 450 nm、EdU阳性细胞率、迁移数升高，细胞凋亡率，上清液中 TNF-α、IL-1β、IL-18水平，细胞中 RhoA、ROCK蛋白表 达水平降低，差异均有统计学意义（P < 0.05）；LPA组足细胞的OD450 nm、EdU阳性细胞率、迁移数低于大黄素高剂量组，细 胞凋亡率，上清液中TNF-α、IL-1β、IL-18水平，细胞中RhoA、ROCK蛋白表达水平高于大黄素高剂量组，差异均有统计 学意义（P < 0.05）。【结论】 大黄素可改善LPS诱导的足细胞损伤，其机制可能与抑制RhoA/ROCK信号通路有关。

Objective To observe the improvement effect and mechanism of emodin on lipopolysaccharide （LPS）-induced podocyte injury. Methods Human glomerular podocytes were used as the study object，and they were randomly dividedintothe blank control group，theLPS group，the emodinlow-，medium-，and high-dose groups， andthe emodin+lysophosphatidic acid [LPA，RhoA/RhoRho-associated coiled-coil kinase（ROCK）activator] group. Cell counting kit 8（CCK8）and 5-ethynyl-2'-deoxyuridine（EdU）staining were applied to detect the proliferation of glomerular podocytes，Transwell assay was used to test the migration of glomerular podocytes，flow cytometry was used to detect apoptosis of glomerular podocytes，and enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of inflammatory factors tumor necrosis factor α （TNF- α），interleukin（IL）-1β， and IL-18 in the supernatant of glomerular podocytes，and the protein expression levels of RhoA and ROCK in glomerular podocytes were determined by Western Blot. Results The optical density（OD）450 nm，EdU positive cell rate and migration number of glomerular podocytes in the LPS group were lower than those in the blank control group，and the apoptosis rate，the levels of TNF-α，IL-1β and IL-18 in the supernatant，as well as the protein expression levels of RhoA and ROCK in the cells were higher than those in the blank control group，the differences being statistically significant（P < 0.05）；the OD450 nm，EdU positive cell rate and migration number of glomerular podocytes in the emodin low-，medium-，and high- dose groups were higher than those in the LPS group，while the apoptosis rate， levels of TNF - α， IL-1β and IL-18 in supernatant， as well as RhoA and ROCK protein expression levels in cells were reduced compared with those in the LPS group，the differences being statistically significant（P < 0.05）；the OD450 nm and EdU positive cell rate and migration number of glomerular podocytes in LPA group were lower than those of emodin high- dose group，while the apoptosis rate，levels of TNF-α，IL-1β and IL-18 in supernatant，as well as RhoA and ROCK protein expression levels in cells were higher than those of emodin high- dose group，the differences being all statistically significant（P < 0.05）. Conclusion Emodin can improve LPS-induced podocyte injury， and its mechanism of action may be related to the inhibition of RhoA / ROCK signaling pathway.

R285.5

河南省医学科技攻关计划（联合共建）项目（编号：LHGJ20220116）