【目的】 基于细胞凋亡 caspase-3 探讨深圳市中医院院内制剂清热益气化瘀中药硬膏对家兔急性软组织损伤的影响。 【方法】 将40只家兔随机分为空白组、模型组、清热化瘀中药硬膏组（简称：原方硬膏组）、清热益气化瘀中药硬膏组[简称： 联合（黄芪）硬膏组]、清热益气化瘀中药浸渍组[简称：联合（黄芪）浸渍组]，每组 8只。除空白组，其他各组家兔采用打击 法构建急性软组织损伤模型。给药后，观察用药 24、72 h 后损伤组织 caspase-3 阳性表达变化以及前列腺素 E2（PGE2） 水平变化。【结果】（1）给药 24 h 后：与空白组比较，3 个给药组与模型组的 caspase-3 阳性表达增强（P < 0.05），其中联 合硬膏组 caspase-3表达最弱，病理评分最低，其次是联合浸渍组、原方硬膏组。给药72 h后：3个治疗组的caspase-3阳性 表达量与空白组比较，差异无统计学意义（P > 0.05），而模型组 caspase-3阳性表达量仍高于空白组（P < 0.05）。（2）给药 24 h 后，模型组血清PGE2水平显著升于空白组（P < 0.05），而3个治疗组血清PGE2水平与空白组、模型组比较，差异无统计学意义 （P > 0.05）。在给药72 h后，原方硬膏组血清PGE2水平与模型组比较，差异无统计学意义（P > 0.05），而联合硬膏组和联合 浸渍组血清 PGE2水平低于模型组（P < 0.05）。【结论】 清热化瘀中药硬膏、清热益气化瘀中药硬膏、清热益气化瘀中药浸渍 均能有效加速急性软组织损伤家兔损伤肌肉组织细胞的凋亡，减轻炎症反应，加速组织损伤的修复，且清热益气化瘀中药 硬膏效果优于清热化瘀中药硬膏、清热益气化瘀中药浸渍。

Objective To explore the effects of Qingre Yiqi Huayu Chinese Medicine Plaster， an hospital preparation of Shenzhen Traditional Chinese Medicine Hospital， on acute soft tissue injury in rabbits from the perspective of the apoptosis caspase-3. Methods Forty rabbits were randomly divided into blank control group， model group，Qingre Huayu Chinese Medicine Plaster group（called original prescription plaster group for short）， Qingre Yiqi Huayu Chinese Medicine Plaster group [called combined（with Astragali Radix）plaster group for short]， and Qingre Huayu Chinese Medicine impregnating group [called combined （with Astragali Radix） impregnating group for short]，with eight rabbits in each group. Except for the blank control group，rabbits in all other groups were used to construct acute soft tissue injury model by striking method. The changes in caspase-3 expression and PGE2 level were observed in the injured tissues after 24-hour and 72-hour drug administration. Results After 24-hour drug administration， compared with the blank control group， the caspase-3 positive expression level of the three treatment groups and the model group were increased（P < 0.05），among which the combined plaster group had the least expression of caspase-3 and the lowest pathological score，followed by the combined impregnating group and the original prescription plaster group. After 72-hour drug administration，the caspase-3 positive expression level of the three treatment groups was not statistically significant compared with that of the blank control group（P > 0.05），while the positive expression level of caspase-3 in the model group was still higher than that of the blank control group（P < 0.05）. After 24-hour drug administration，the serum PGE2 level of the model group was significantly higher than that of the blank control group（P < 0.05），while the difference of the serum PGE2 levels between the three treatment groups and the blank control group or the model group being not statistically significant（P > 0.05）；after 72-hour drug administration，the difference of the serum PGE2 level between the original prescription plaster group and the model group being not statistically significant（P > 0.05）， while the serum PGE2 level of the combined plaster group and combined impregnating group was lower than that of the model group，respectively（P < 0.05）. Conclusion All of Qingre Huayu Chinese Medicine Plaster，Qingre Yiqi Huayu Chinese Medicine Plaster， Qingre Yiqi Huayu Chinese Medicine impregnation can effectively accelerate the apoptosis of injured muscle tissue cells in rabbits with acute soft tissue injury，reduce inflammatory reaction and accelerate the repair of tissue damage，and the effect of Qingre Yiqi Huayu Chinese Medicine Plaster was superior to that of Qingre Huayu Chinese Medicine Plaster and Qingre Yiqi Huayu Chinese Medicine impregnation.

R285.5

深圳市科技创新委员会项目（编号：JCYJ20180302173610088）