【目的】 观察电针阳陵泉和曲池穴对大鼠脑卒中肢体痉挛的治疗作用及机制。【方法】 将大鼠随机分为假手术组、模型 组、电针组和电针+CA1[Janus激酶 2（JAK2）激动剂]组。除假手术组，其余各组大鼠采用改良的大脑中动脉线栓联合内囊注 射N-甲基-D-天冬氨酸（NMDA）受体法制备脑卒中肢体痉挛模型。干预后，对大鼠进行神经功能缺损评分、阿什沃斯肌肉痉 挛评定量表（Ashworth）评分和电生理测定；酶联免疫吸附分析（ELISA）检测脑组织中白细胞介素 6（IL-6）、肿瘤坏死因子 α （TNF-α）、γ-氨基丁酸（GABA）水平；采用比色法检测谷氨酸（Glu）含量；苏木素-伊红（HE）染色法观察脑组织病理学变 化；Western Blot 法检测脑组织中 JAK2、磷酸化 JAK2（p-JAK2）、信号转导子和转录激活子 3（STAT3）、磷酸化 STAT3 （p-STAT3）蛋白表达。【结果】 模型组神经功能缺损评分，Ashworth评分，脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、 p-STAT3/STAT3 比值均显著高于假手术组（P < 0.05），肌张力信号值、脑组织中 GABA 含量显著低于假手术组（P < 0.05）； 与模型组比较，电针组神经功能缺损评分，Ashworth评分，脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、p-STAT3/STAT3 比值均显著降低（P < 0.05），肌张力信号值、脑组织中 GABA 含量显著升高（P < 0.05）；与电针组比较，电针+CA1 组神经 功能缺损评分，Ashworth评分，脑组织中IL-6、TNF-α、Glu含量及p-JAK2/JAK2、p-STAT3/STAT3比值显著升高（P < 0.05）， 肌张力信号值、脑组织中GABA含量显著降低（P < 0.05）。【结论】 电针可能通过抑制JAK2/STAT3通路抑制炎症反应，调节神 经元兴奋/抑制平衡，从而缓解脑卒中大鼠肢体痉挛。

Objective To observe the therapeutic effect and mechanism of electroacupuncture at Yanglingquan （GB34）and Quchi（LI11）points for post-stroke spasticity（PSS）in rats. Methods The rats were randomly divided into sham-operated group，model group，electroacupuncture group and electroacupuncture + CA1 [Janus kinase 2 （JAK2）agonist］ group. The PSS model was prepared by the modified middle cerebral artery occlusion combined with internal capsule injection of N-methyl-D-aspartate（NMDA）receptor method in all groups of rats except the sham-operated group. After the intervention， the neurological deficit scale scoring， Ashworth Spasticity Scale （Ashworth）scoring and electrophysiological determination were carried out；the levels of interleukin 6（IL-6），tumor necrosis factor α（TNF-α）and γ-aminobutyric acid（GABA）in the brain tissues were detected by enzymelinked immunosorbent assay（ELISA）；the content of glutamate（Glu）was observed by colorimetry；the pathological changes in brain tissues were observed by hematoxylin-eosin（HE）staining method；the protein expression levels of JAK2， phosphorylated JAK2（p-JAK2）， signal transducer and activator of transcription 3（STAT3）， and phosphorylated STAT3 （p-STAT3） in brain tissues were detected by Western Blot method. Results The neurological deficit scale scores，Ashworth scores，contents of IL-6，TNF-α and Glu as well as p-JAK2/JAK2 and p-STAT3/STAT3 ratios in brain tissues in the model group were all significantly higher than those in the shamoperated group（P < 0.05），and the muscular tension signal value and GABA content in brain tissues in the model group were significantly lower than those in the sham-operated group（P < 0.05）；compared with the model group， the neurological deficit scale scores，Ashworth scores，contents of IL-6，TNF-α and Glu as well as p-JAK2/ JAK2 and p-STAT3/STAT3 ratios in brain tissues were significantly lower in the electroacupuncture group（P < 0.05），and the muscular tension signal value and GABA content in brain tissues were significantly higher in the electroacupuncture group（P < 0.05）；compared with the electroacupuncture group，the neurological deficit scale scores，Ashworth scores，contents of IL-6，TNF - α and Glu as well as p-JAK2 / JAK2 and p-STAT3 / STAT3 ratios in brain tissues in the electroacupuncture + CA1 group were significantly higher（P < 0.05）， and the muscular tension signal value and GABA content in brain tissues in the electroacupuncture + CA1 group were significantly lower（P < 0.05）. Conclusion Electroacupuncture may inhibit inflammatory responses and regulate the neuronal excitation/inhibition balance by inhibiting the JAK2/STAT3 pathway，thus relieving PSS in rats.

R245.31

黑龙江省卫生健康委科研课题（编号：20230303100276）