【目的】 观察丹黄散含药血清对高糖诱导的血管内皮细胞损伤的修复作用及其机制。【方法】 制备丹黄散含药血清。 培养人脐静脉血管内皮细胞（HUVECs），将其分为对照组、重组人表皮生长因子（简称生长因子）组、丹黄散组、高糖组、高 糖+生长因子组、高糖+丹黄散组。各组对应处理48 h后，应用透射电镜观察细胞超微结构及自噬情况，流式细胞仪检测细 胞凋亡，Western Blot法检测细胞中血管内皮生长因子（VEGF）、表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的蛋 白表达水平。【结果】（1）对照组细胞线粒体内脊清晰可见，自噬体及自噬溶酶体较少见；高糖组线粒体肿胀、不规则，出 现空泡化；高糖＋丹黄散组可见较多典型自噬样结构。（2）与高糖组、高糖+生长因子组比较，高糖+丹黄散组细胞凋亡率显 著降低（P < 0.05）。（3）与高糖组、高糖+生长因子组比较，高糖+丹黄散组细胞中VEGF、EGF、bFGF 的蛋白表达水平显著升 高（P < 0.05）。【结论】 丹黄散含药血清可减轻高糖对HUVEC细胞的损伤，其机制可能与激活线粒体自噬，抑制细胞凋亡以 及上调VEGF、EGF、bFGF的表达有关。

Objective To observe the repair effect and mechanism of Danhuang Powder-containing serum on high glucose - induced vascular endothelial cell injury. Methods Danhuang Powder-containing serum was prepared. Human umbilical vein endothelial cells（HUVECs）were cultured to be divided into control group，recombinant human epidermal growth factor（called “growth factor” for short）group， Danhuang Powder group， high glucose group，high glucose+growth factor group，and high glucose+Danhuang Powder group. After corresponding intervention in each group for 48 hours，the cell ultrastructure and autophagy were observed under transmission electron microscope， apoptosis was detected by flow cytometry， and the protein expression levels of vascular endothelial growth factor（VEGF），epidermal growth factor（EGF）and basic fibroblast growth factor（bFGF）in the cells were detected by Western Blot. Results（1）The intra-mitochondrial ridges in the control group were clearly visible， autophagosomes and autolysosomes were fewer； mitochondria in the high glucose group were swollen and irregular，and appeared vacuolated；and the more typical autophagy-like structures were seen in the high glucose+Danhuang Powder group.（2）Compared with the high glucose group and high glucose+growth factor group，the apoptosis rate of cells in the high glucose+ Danhuang Powder group was significantly decreased（P < 0.05）.（3） Compared with the high glucose group and the high glucose + growth factor group， the protein expression levels of VEGF， EGF and bFGF in the cells of the high glucose + Danhuang Powder group were significantly increased（P < 0.05）. Conclusion Danhuang Powder-containing serum can reduce the high glucoseinduced damage in HUVEC cells，and its mechanism may be related to the activation of mitochondrial autophagy， and the inhibition of apoptosis，as well as the up-regulation of the expression of VEGF，EGF and bFGF.

R285.5

国家自然科学基金资助项目（编号：81960805）