【目的】观察化瘀消癥颗粒对miR-210过表达与低氧状态下滋养细胞血管生成、侵袭的影响。 【方法】体外培养HTR-8/SVneo细胞，通过转染建立miR-210 过表达模型，以CoCl2法建立化学低氧模型，将实验分为空白组、miR-210过表达组、miR-210过表达阴性组、miR-210过表达+中药组、低氧+中药组及中药组，采用Western Blot法检测各组HTR-8/SVneo 细胞 中缺氧诱导因子1α （HIF-1α） 、血管生成因子 （VEGF） 、侵袭相关因子[基质金属蛋白酶9 （MMP-9） 、金属蛋白酶组织抑制剂1（TIMP-1） ]蛋白的表达。 【结果】 与空白组比较，miR-210过表达组中HIF-1α、VEGF、MMP-9、TIMP-1蛋白表达水平及MMP-9/TIMP-1比值升高 （P＜0.05） ；与miR-210过表达组比较，miR-210过表达阴性组、miR-210 过表达+中药组、低氧+中药组、中药组中HIF-1α、VEGF、MMP-9、TIMP-1蛋白表达水平及MMP-9/TIMP-1比值均下降 （P＜0.05） ，中药组下降作用尤其明显；与miR-210 过表达+中药组比较，低氧+中药组、中药组中HIF-1α、VEGF、MMP-9、TIMP-1蛋白表达水平及MMP-9/TIMP-1比值下降 （P＜0.05） ；与低氧+中药组比较，中药组上述各指标均下降 （P＜0.05） 。 【结论】化瘀消癥颗粒对miR-210 过表达及低氧状态下滋养细胞所增强的血管生成、侵袭能力具有负调控作用，这可能是其治疗输卵管妊娠的作用机制之一。

Objective To observe the effect of Huayu Xiaozheng Granules on angiogenesis and invasion in trophoblast cells with overexpression of miR-210 and under hypoxia. Methods HTR-8/SVneo cells were cultured in vitro，a miR-210 overexpression model was established by transfection，and a chemical hypoxia model was established by CoCl2 method. The blank group， miR-210 overexpression group， miR-210 overexpression-negative group， miR-210 overexpression+Chinese medicine group， hypoxia+Chinese medicine group and Chinese medicine group in experiment were set up. Western Blot method was used to detect protein expressions of hypoxia-inducible factor 1α （HIF-1α），vascular endothelial growth factor （VEGF），and invasion-associated factors [matrix metalloproteinase 9 （MMP-9） and tissue inhibitors of matrix metalloproteinase 1 （TIMP-1） ] in HTR-8/SVneo cells. Results Compared with the blank group，the protein expression levels of HIF-1α，VEGF，MMP-9，TIMP-1 and MMP-9/TIMP-1 ratio were increased in the miR-210 overexpression group （P＜0.05）；compared with the miR-210 overexpression group，the HIF-1α，VEGF，MMP-9，TIMP-1 protein expression levels and MMP-9/TIMP-1 ratio in the miR-210 overexpression-negative group， miR-210 overexpression+Chinese medicine group，hypoxia+Chinese medicine group and Chinese medicine group were all decreased（P＜0.05） ，and the decrease effects in Chinese medicine group was especially obvious；compared with the miR-210 overexpression+Chinese medicine group，the protein expression levels of HIF-1α，VEGF，MMP-9，TIMP-1 and MMP-9/TIMP-1 ratio in hypoxia+Chinese medicine group and Chinese medicine group were all decreased （P＜0.05） ；compared with the hypoxia+Chinese medicine group，the above indicators were decreased in the Chinese medicine group（P＜0.05） . Conclusion Huayu Xiaozheng Granules can negatively regulate the increased angiogenesis and invasion of trophoblast cells with miR-210 overexpression and under hypoxia，which may be one of the action mechanisms of its treatment for tubal pregnancy.

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国家自然科学基金面上项目 （编号：81804134，82174417）；广东省中医药局面上项目 （编号：20231172）；广州市科学技术局市校联合资助项目 （编号：202201020326）