【目的】观察补肾健脾中药复方（振元颗粒）对雌激素缺乏所致小鼠超重的干预效果及机制。【方法】构建卵巢摘除雌性小鼠模型，观察连续14周灌胃振元颗粒对小鼠体质量和脂肪堆积的影响。构建小鼠3T3-L1前脂肪细胞模型，观察振元颗粒干预对成脂分化的影响。检测小鼠脂肪组织和3T3-L1细胞中调控脂肪细胞形成和脂肪合成的关键基因/蛋白的表达情况，探讨振元颗粒体内外减脂作用的机制。【结果】振元颗粒干预显著降低卵巢摘除小鼠的体质量增量（P＜0.01）、性腺周围和腹股沟脂肪指数（P＜0.05），显著抑制前脂肪细胞向脂肪细胞的分化，显著下调脂肪组织和3T3-L1细胞中过氧化物酶体增殖物激活受体γ（PPARγ）、胆固醇调节元件结合蛋白1c（SREBP-1c）、乙酰辅酶A羧化酶1（ACC1）和脂肪酸合酶（FAS）表达水平（P＜0.05），显著提高腺苷酸活化蛋白激酶（AMPK）和ACC1的磷酸化水平（P＜0.05）。【结论】振元颗粒能抑制脂肪细胞生成，改善雌激素缺乏造成的雌性小鼠超重，其机制与激活AMPK信号通路有关。提示补肾健脾中药复方振元颗粒有助于绝经后女性的体质量控制。

Objective To observe the intervention effect and mechanism of Chinese herbal compound （Zhenyuan Granules）on overweight in mice caused by estrogen deficiency. Methods An ovariectomized female mouse model was established to observe the effects of intragastric 14 week administration of Zhenyuan Granules on body mass and fat accumulation in mice. The 3T3-L1 mouse preadipocyte model was constructed to observe the effect of Zhenyuan Granules intervention on adipogenic differentiation. The expressions of key genes/proteins regulating adipocyte formation and fat synthesis in mouse adipose tissue and 3T3-L1 cells was detected to explore the mechanism of lipid-lowering effect of Zhenyuan Granules in vivo and in vitro. Results Zhenyuan Granules significantly decreased the body mass gain（P＜0.01），perigonadal and inguinal fat indexes（P＜0.05），significantly inhibited the differentiation of 3T3-L1 preadipocytes into mature adipocytes（P＜0.01），significantly downregulated the expression levels of transcription factors peroxisome proliferators activated receptor γ（PPARγ）and sterol-regulatory element binding protein 1c（SREBP-1c）and enzymes acetyl-CoA carboxylase 1（ACC1）and fatty acid synthase（FAS） in adipose tissue and 3T3-L1 preadipocytes（P＜0.05），significantly enhanced the phosphorylation of AMP-activated protein kinase（AMPK）and ACC1（P＜0.05）. Conclusion Zhenyuan Granules can inhibit the formation of adipocytes and improve the overweight of female mice caused by estrogen deficiency. The mechanism is related to the activation of AMPK signaling pathway. It is suggested that Bushen Jianpi Chinese herbal compound Zhenyuan Granules is helpful for body mass control in postmenopausal women.

R285.5

国家重点研发计划项目 （编号：2019YFE0109800）；广州中医药大学学科协同创新团队项目 （编号：2021-XK-75）