【目的】探讨苍附导痰汤对肥胖型多囊卵巢综合征 （PCOS） 大鼠的治疗作用及机制。 【方法】将15只雌性SD大鼠随机分 为正常组、模型组、中药组，每组5只。除正常组外，其余大鼠采用来曲唑溶液灌胃联合高脂饲料喂养法构建肥胖型PCOS 模型。造模成功后，中药组大鼠给予苍附导痰汤灌胃30 d。给药结束后，采用非标蛋白质组学定量技术检测各组大鼠卵巢蛋 白表达，比较组间差异蛋白功能、基因本体论 （GO） 富集、京都基因与基因组百科全书 （KEGG） 富集及差异蛋白KEGG通路聚 类结果。 【结果】模型组与正常组间差异蛋白质的功能主要集中在脂质转运、代谢和翻译后修饰、蛋白转录方面，KEGG通路 富集及通路富集的聚类分析结果主要集中在缬氨酸、亮氨酸和异亮氨酸降解，精氨酸和脯氨酸代谢、色氨酸代谢通路富集 及肾素血管紧张素系统；中药组与模型组间差异蛋白的功能集中在信息储存及处理，尤其是转化、核糖体结构和信号转导 机制方面，KEGG通路富集及通路富集的聚类分析结果主要集中在核糖体代谢、药物的代谢-细胞色素酶P450、丁酸甲酯代 谢、维生素B6代谢方面。中药组与正常组间差异蛋白功能分类主要在信号转导机制、脂质转运和代谢方面，KEGG通路富 集及通路富集的聚类分析结果主要集中在核糖体、蛋白的消化和吸收、类固醇激素生物合成通路、细胞黏附分子、甘油脂 类代谢、胃酸分泌方面。 【结论】苍附导痰汤可能通过调节支链氨基酸代谢、肾素-血管紧张素-醛固酮系统、类固醇激素合 成通路起到治疗肥胖型PCOS的作用。

Objective To analyze the therapeutic effect and mechanism of Cangfu Daotan Decoction on obese polycystic ovary syndrome （PCOS） rats. Methods Fifteen female SD rats were randomly divided into normal group， model group and Chinese medicine group，with five rats in each group. In addition to the normal group，the remaining rats were treated with Letrozole Solution by gavage combined with high-fat diet to construct an obese PCOS model. After successful modeling， the rats in the Chinese medicine group were given Cangfu Daotan Decoction by gavage for 30 days. At the end of administration，the ovarian protein expression of rats in each group was detected by non-standard proteomics quantitative technique，and the results of differential protein function， gene ontology （GO） enrichment，Kyoto Encyclopedia of Genes and Genomes（KEGG） enrichment and differential protein KEGG pathway clustering were compared. Results The functions of differential proteins between the model group and the normal group were mainly concentrated in lipid transport， metabolism and post-translational modification， and protein transcription， and the cluster analysis results of KEGG pathway enrichment and pathway enrichment were mainly concentrated in the degradation of valine，leucine and isoleucine，arginine and proline metabolism，tryptophan metabolism pathway enrichment and renin angiotensin system. The functions of differential proteins between the Chinese medicine group and the model group were concentrated in information storage and processing，especially in transformation，ribosomal structure and signal transduction mechanism， and the cluster analysis results of KEGG pathway enrichment and pathway enrichment were mainly concentrated in ribosome metabolism， drug metabolism-cytochrome P450， methyl butyrate metabolism， and vitamin B6 metabolism. The functional classification of differential proteins between Chinese medicine group and normal group was mainly in signal transduction mechanism，lipid transport and metabolism，and the clustering analysis results of KEGG pathway enrichment and pathway enrichment were mainly concentrated in ribosome，protein digestion and absorption，steroid hormone biosynthesis pathway，cell adhesion molecule，glycerol lipid metabolism and gastric acid secretion. Conclusion Cangfu Daotan Decoction may play a role in the treatment of obese PCOS by regulating branched-chain amino acid metabolism，renin-angiotensin-aldosterone system and steroid hormone synthesis pathway.

R285.5

国家自然科学基金青年科学基金资助项目 （编号：82104913，81904233）；全国名老中医药专家黄健玲名医传承工作室 （国中 医药人教函〔2022〕75号）；广东省中医药局科研项目面上项目 （编号：20221176，20201150）；广东省中医院朝阳人才课题 （编号： ZY2022KY04）