【目的】 观察足三里-中脘合募配穴针刺对运动应激性胃溃疡大鼠的治疗作用及机制。 【方法】 将40只雄性SD大鼠随机 分为空白组、模型组、针刺组、奥美拉唑组，每组10只。除空白组外，其余各组大鼠均采用每日力竭游泳法构建运动应激 性胃溃疡模型。成功造模后，针刺组选用足三里穴和中脘穴针刺干预，每日1次，每次10 min；奥美拉唑组大鼠每日游泳 2 h前给予奥美拉唑肠溶片蒸馏水混悬液灌胃。连续干预7 d后，观察大鼠整体状态与行为学，Guth法计算胃黏膜损伤指数， 苏木素-伊红 （HE） 染色法观察胃黏膜病理形态，采用WST-1法、比色法和TBA法分别对应测定血清超氧化物歧化酶 （SOD） 、 谷胱甘肽过氧化物酶 （GSH-PX） 和丙二醛 （MDA） 含量，酶联免疫吸附分析 （ELISA） 检测血清中胃泌素 （GAS） 、生长抑素 （SS） ， 肿瘤坏死因子α （TNF-α） 、白细胞介素1β （IL-1β） 、白细胞介素6 （IL-6） 和白细胞介素10 （IL-10） 的含量，实时荧光定量聚合 酶链反应 （qPCR） 法检测胃黏膜组织表皮生长因子受体 （EGFR） 、基质金属肽酶3 （MMP3） 、核因子红细胞系相关因子2 （NRF2） 、血红素加氧酶1 （HO-1） 和线粒体超氧化物歧化酶2 （SOD2） 、TNF-α、IL-1β、IL-6和IL-10 mRNA表达水平。 【结果】与空白组比较，模型组大鼠体质量增长缓慢，旷场试验中活动路程缩短、活跃度降低，胃黏膜溃疡指数显著升高， 胃黏膜功能指标血清GAS水平升高、血清SS水平降低、胃黏膜组织中EGFR mRNA表达水平降低及胃黏膜组织MMP3 mRNA 表达水平升高，血清中抗氧化物质SOD、GSH-PX水平显著降低，氧化产物MDA水平显著升高，胃黏膜组织中抗氧化基 因NRF2、HO-1、SOD2 mRNA表达水平显著降低，炎症因子TNF-α、IL-1β、IL-6的血清含量与胃黏膜组织mRNA水平 显著升高，IL-10的血清含量与胃黏膜组织mRNA水平显著降低 （P＜0.05或P＜0.01或P＜0.001） ，HE染色可见明显胃黏膜 损伤；与模型组比较，针刺组、奥美拉唑组上述各指标均得到明显改善 （P＜0.05或P＜0.01或P＜0.001） ，HE染色可见胃黏 膜损伤明显减轻。 【结论】足三里-中脘合募配穴针刺可减轻运动应激性胃溃疡大鼠局部的氧化应激和炎症反应，减少胃黏膜 损伤，改善大鼠情绪状态，维持大鼠整体活力。

Objective To observe the therapeutic effect and mechanism of acupuncture at Zusanli-Zhongwan combined matching points on rats with exercise-induced stress gastric ulcer. Methods Forty male SD rats were randomly divided into blank group，model group，acupuncture group and Omeprazole group，with 10 rats in each group. Except for the blank group，the rats in the other groups were used to construct the model of exercise stress gastric ulcer by daily exhaustive swimming. After successful modeling，the acupuncture group was intervened by acupuncture at Zusanli（ST36） and Zhongwan（RN12）， once a day， 10 minutes each time. Rats in the Omeprazole group were given Omeprazole Enteric-Coated Tablets distilled water suspension by gavage two hours before daily swimming. After continuous 7-day intervention，the overall state and behavior of rats were observed， the gastric mucosal injury index was calculated by Guth method，the pathological morphology of gastric mucosa was observed by hematoxylin-eosin （HE） staining，the contents of superoxide dismutase （SOD），glutathione peroxidase （GSH-PX） and malondialdehyde （MDA） in serum were correspondingly determined by WST-1 method，colorimetry and TBA method，respectively，enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of gastrin （GAS），somatostatin （SS），tumor necrosis factor-α （TNF-α），interleukin-1β （IL-1β） ，interleukin-6 （IL-6） and interleukin-10 （IL-10） in serum，the expression levels of epidermal growth factor receptor （EGFR） ，matrix metalloproteinase 3 （MMP3） ，nuclear factor erythroid-related factor 2 （NRF2） ， heme oxygenase 1 （HO-1） and mitochondrial SOD2，TNF-α，IL-1β，IL-6 and IL-10 mRNA in gastric mucosa were detected by real-time fluorescence quantitative polymerase chain reaction （qPCR） . Results Compared with the blank group，the body mass of rats in the model group was increased slowly，the activity distance and activity in the open field test were decreased，the gastric mucosal ulcer index was increased significantly，the gastric mucosal function indexes involving GAS level was increased and SS level was decreased in serum，the mRNA expression level of EGFR in gastric mucosa was decreased and the mRNA expression level of MMP3 in gastric mucosa was increased. The serum levels of antioxidant substances SOD and GSH-PX were decreased significantly， and the serum level of oxidation product MDA was increased significantly. The mRNA expression levels of antioxidant genes NRF2，HO-1 and SOD2 in gastric mucosa were significantly decreased. The serum contents and the gastric mucosa mRNA levels of inflammatory factors TNF- α，IL-1α，IL-6 were significantly increased， while the serum content and the gastric mucosa mRNA level of IL-10 were significantly decreased.The differences were statistically significant （P＜0.05 or P＜0.01 or P＜0.001） . HE staining showed obvious gastric mucosal injury. Compared with the model group，the above indexes in the acupuncture group and the Omeprazole group were significantly improved （P＜0.05 or P＜0.01 or P＜0.001） . HE staining showed that the gastric mucosal injury was significantly reduced. Conclusion Acupuncture at Zusanli-Zhongwan combined matching points can reduce the local oxidative stress and inflammatory response in rats with exercise-induced stress gastric ulcer，reduce gastric mucosal injury，improve the emotional state of rats，and maintain the overall vitality of rats.

R285.5

广东省教育厅青年创新人才项目 （编号：2021KQNCX185）；广东省方证研究重点实验室项目 （编号：2022B1212010012）；广东 食品药品职业学院自然科学项目 （编号：2021 ZR10）