【目的】 建立广陈皮的高效液相色谱（HPLC）陈化特征图谱。【方法】 采用HPLC-二极管阵列检测器（DAD）技术分析不同 年份广陈皮之间的成分差异，测定各差异色谱峰的相对峰面积，通过聚类分析对其进行陈化等级分类，并通过中药色谱指 纹图谱相似度评价系统（2012A 版）进行评价。【结果】 不同年份广陈皮被聚为Ⅰ、Ⅱ类两个不同的陈化等级，Ⅰ类（低陈化等 级）广陈皮的相似度为 0.708 ~ 0.957，Ⅱ类（高陈化等级）广陈皮的相似度为 0.734 ~ 0.976，且两者之间的相似度均低于 0.6。 建立的特征图谱中存在 9 个差异色谱峰；Ⅱ类广陈皮的对照特征图谱 T2 可视为其陈化特征图谱，其中 1 ~ 4号峰、9号峰 为陈化特征峰。【结论】 建立的广陈皮陈化特征图谱操作简单，具有良好的重复性与可靠性，可用于不同年份广陈皮的质量 评价。

Objective To establish a high performance liquid chromatography（HPLC） aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium. Methods HPLC-diode array detector（DAD）was used to analyze the composition differences between different years of Guangdong Citri Reticulatae Pericarpium，and the relative peak area of each differential chromatographic peak was determined. The aging grade was classified by cluster analysis，and evaluated by the similarity evaluation system for chromatographic fingerprint of TCM（2012A edition）. Results The different years of Guangdong Citri Reticulatae Pericarpium was clustered into two different aging grades of class Ⅰ and class Ⅱ . The similarity of class Ⅰ（low aging grade）Guangdong Citri Reticulatae Pericarpium was 0.708-0.957， and the similarity of class Ⅱ（high aging grade）Guangdong Citri Reticulatae Pericarpium was 0.734- 0.976， and the similarity between the two was lower than 0.6. There were 9 different chromatographic peaks in the established characteristic chromatogram. The control characteristic spectrum T2 of type Ⅱ Guangdong Citri Reticulatae Pericarpium can be regarded as its aging characteristic spectrum， among which peaks 1-4 and 9 are aging characteristic peaks. Conclusion The established aging characteristic chromatogram of Guangdong Citri Reticulatae Pericarpium is simple in operation， with good repeatability and reliability，and can be used for quality evaluation of different years of Guangdong Citri Reticulatae Pericarpium.

R282.5

国家药品监督管理局药品快速检验技术重点实验室开放性课题（编号：KF2021005）；广州市科技计划项目（编号：201710010122）