【目的】 观察姜黄素对正畸牙齿移动（OTM）的作用及其潜在机制。【方法】 将 30只大鼠随机分为 5组，即正常组，模型 组，姜黄素40、80、160 mg/kg组，每组6只。除正常组，其余各组大鼠均构建OTM模型。干预后，微型CT断层扫描上颌骨 观察位移，苏木素-伊红（HE）染色法观察上颌组织病理形态，末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染 色法观察牙周膜组织细胞凋亡，采用流式细胞术测定牙周膜活性氧簇（ROS）水平，酶标仪测量牙周膜组织丙二醛（MDA）含量 及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，分别采用免疫荧光染色法、Western Blot法检测牙周膜组织 中糖原合成酶激酶3β（GSK-3β）/β-连环蛋白（β-catenin）通路相关蛋白表达和自噬水平。【结果】 与模型组比较，姜黄素各剂 量组 OTM 诱导的牙周膜成纤维细胞增多，牙周膜中 MDA 和 ROS 水平降低， SOD 和 GSH-Px 活性升高，牙周膜自噬指标 Beclin1、Lamp 和 LC3B 的蛋白表达水平降低，牙周膜 p-GSK-3β/GSK-3β 和 β-catenin 蛋白表达水平降低（均 P＜0.05）。 【结论】 姜黄素可能通过阻断GSK-3β/β-catenin通路抑制氧化应激，从而抑制正畸牙齿移动过程中牙周膜自噬。

Objective To observe the effect of curcumin on orthodontic tooth movement（OTM）and its potential mechanism. Methods Thirty rats were randomly divided into 5 groups：normal group，model group，curcumin 40，80，160 mg/kg groups，with 6 rats in each group. In addition to the normal group，the other groups of rats were constructed OTM model. After intervention， the displacement of maxilla was observed by micro-CT tomography. The pathological morphology of maxillary tissue was observed by hematoxylin-eosin（HE）staining. The apoptosis of periodontal ligament cells was observed by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling（TUNEL）staining. The level of reactive oxygen species（ROS）in periodontal ligament was determined by flow cytometry. The content of malondialdehyde（MDA）and the activities of superoxide dismutase （SOD）and glutathione peroxidase（GSH-Px）in periodontal ligament tissue were measured by microplate reader. Immunofluorescence staining and Western Blot were used to detect the expressions of glycogen synthase kinase 3β （GSK-3β）/β -catenin pathway-related proteins and autophagy levels in periodontal ligament tissues. Results Compared with the model group，the number of OTM-induced periodontal ligament fibroblasts was increased，the levels of MDA and ROS in periodontal ligament were decreased， the activities of SOD and GSH-Px were increased， the protein expressions of autophagic indexes including Beclin1， Lamp and LC3B in periodontal ligament were decreased，and the expressions of p-GSK-3β/GSK-3β and β-catenin in periodontal ligament was decreased in curcumin groups（all P＜0.05）. Conclusion Curcumin may inhibit oxidative stress by blocking the GSK-3β/β-catenin pathway，thereby inhibiting periodontal membrane autophagy during orthodontic tooth movement.

R285.5

福建省卫生健康科技计划项目（编号：2020CXB053）