【目的】 建立制何首乌提取液多成分含量测定方法，比较制何首乌煮散饮片与传统饮片煎煮过程中化学成分的变化规 律，为中药煮散饮片的制备和临床应用提供科学依据。【方法】 采用高效液相色谱法（HPLC）测定没食子酸、5-羟甲基糠醛、 2，3，5，4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素等 主要成分的含量，以出膏率、醇溶性浸出物作为评价指标，考察标准汤剂煎煮法和非标准汤剂煎煮法对制何首乌煮散饮片 和原饮片提取液的影响。【结果】 非标准汤剂煎煮法：制何首乌煮散饮片中没食子酸、5-羟甲基糠醛、2，3，5，4’-四羟基二苯 乙烯-2-O-β-D-葡萄糖苷、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素等指标性成分含量均显 著性提高，提升比例分别为 71.18%、141.67%、146.29%、150.00%、625.00%、296.00%，尤其是大黄素甲醚-8-O-β-D-葡 萄糖苷提升了7.25倍。标准汤剂煎煮法：制何首乌煮散饮片中上述指标性成分含量均显著性提高，提升比例分别为28.09%、 10.53%、66.01%、45.45%、316.67%、65.85%，尤其是大黄素甲醚-8-O-β-D-葡萄糖苷提升了 4.17 倍。采用标准汤剂煎煮 法的煮散饮片各指标含量的相对标准偏差（RSD）在10%以内。【结论】 制何首乌煮散饮片指标性成分溶出和质量均一性均优于 原饮片，可提高临床用药的精准性，需酌情减量使用。

Objective To establish a method for the determination of multi-component content of prepared Polygoni Multiflori Radix extract，and to compare the changes of chemical components in the decoction process of prepared Polygoni Multiflori Radix powder decoction pieces and traditional decoction pieces，so as to provide scientific basis for the preparation and clinical application of traditional decoction pieces. Methods The contents of gallic acid， 5-hydroxymethylfurfural，2，3，5，4’-tetrahydroxystilbene-2-O-β-D-glucoside，emodin-8-O-β-D-glucoside， physcion-8-O-β-D-glucoside and emodin were determined by high performance liquid chromatography（HPLC）. With the extraction rate and alcohol-soluble extract as evaluation indexes， the effects of standard decoction method and non-standard decoction method on the extraction of prepared Polygoni Multiflori Radix powder decoction pieces and original decoction pieces were investigated. Results Non-standard decoction boiling method： the contents of gallic acid， 5-hydroxymethylfurfural， 2，3，5，4’-tetrahydroxystilbene-2-O- β -D-glucoside， emodin-8-O- β -D-glucoside， physcion-8-O- β -D-glucoside and emodin in the prepared Polygoni Multiflori Radix powder decoction pieces were significantly increased， and the increase ratios were 71.18%， 141.67%， 146.29%， 150.00%， 625.00% and 296.00%， respectively. In particular， physcion-8-O- β -D-glucoside increased by 7.25 times. Standard decoction boiling method： the content of the above index components in the prepared Polygoni Multiflori Radix powder decoction pieces was significantly increased，and the increase ratios were 28.09%，10.53%，66.01%，45.45%，316.67%，and 65.85%，respectively. In particular，physcion-8- O-β-D-glucoside increased by 4.17 times. The relative standard deviation（RSD）of each index content of prepared powder decoction by standard decoction boiled method was within 10%. Conclusion The dissolution and quality uniformity of the index components of the prepared Polygoni Multiflori Radix powder decoction pieces are superior to those of the original decoction pieces，which can improve the accuracy of clinical medication and need to be reduced as appropriate.

R283

广东省中医药局科研项目（编号：20212087）