【目的】 观察虎杖苷对宫腔粘连大鼠的治疗作用及机制。【方法】 从40只可育雌性SD大鼠中随机选择8只作为假手术组， 剩余 32只大鼠采用宫内机械损伤联合脂多糖感染法构建宫腔粘连模型。将 32只成模大鼠随机分为模型组，虎杖苷低、中、 高剂量组，每组8只，连续相应干预14 d。苏木素-伊红（HE）染色法观察子宫内膜形态结构，测量子宫内膜厚度并计数子宫 内膜腺体数量；Masson 染色法观察子宫内膜纤维化情况；酶联免疫吸附分析（ELISA）检测子宫内膜组织中肿瘤坏死因子 α （TNF-α）、白细胞介素（IL）-1β、IL-6 含量；Western Blot 法检测子宫内膜组织 Wnt/β-连环蛋白（β-catenin）信号通路关键 蛋白 β-catenin、糖原合成酶激酶3β（GSK-3β）、磷酸化糖原合成酶激酶3β（p-GSK-3β）、Cyclin D1及纤维化相关蛋白转化生 长因子 β1（TGF-β1）、α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原蛋白（Col Ⅰ）蛋白及Ⅲ型胶原蛋白（Col Ⅲ）的蛋白表达量。 【结果】 与假手术组比较，模型组及虎杖苷低、中、高剂量组大鼠子宫内膜组织可见不同程度的病理损伤，内膜厚度及腺体 数量降低，并可见不同程度的胶原纤维沉积，子宫内膜组织中TNF-α、IL-1β、IL-6水平，β-catenin、Cyclin D1蛋白相对表 达量及GSK-3β蛋白活化水平，TGF-β1、α-SMA、Col Ⅰ及Col Ⅲ蛋白相对表达量均升高（P＜0.05）；与模型组比较，虎杖苷 低、中、高剂量组大鼠子宫内膜组织病理损伤改善，内膜厚度及腺体数量增加，内膜纤维化程度减轻，子宫内膜组织中上 述指标因子水平均降低（P＜0.05），且呈剂量依赖性。【结论】 虎杖苷可促进宫腔粘连大鼠子宫内膜修复并抑制内膜过度纤维 化，其作用机制可能与抑制Wnt/β-catenin信号通路活化有关。

Objective To observe the therapeutic effect and mechanism of polydatin on intrauterine adhesions （IUA）in rats. Methods Eight rats were randomly selected as the sham operation group from 40 fertile female SD rats， and the remaining 32 rats were given intrauterine mechanical injury combined with lipopolysaccharide infection to induce IUA model. The 32 model rats were randomly divided into model group，low-，medium- and high- dose groups of polydatin，with 8 rats in each group，and the corresponding intervention lasted for 14 days. The morphological structure of endometrium was observed by hematoxylin-eosin（HE）staining，the thickness of endometrium was measured and the number of endometrial glands was counted. Masson staining was used to observe endometrial fibrosis； enzyme-linked immunosorbent assay（ELISA）was used to detect the contents of tumor necrosis factor-α（TNF-α），interleukin（IL）-1β and IL-6 in endometrial tissue. Western Blot was used to detect the ptotein expressions of key proteins of Wnt/β-catenin signaling pathway，including β-catenin，glycogen synthase kinase 3β（GSK-3β），phosphorylated glycogen synthase kinase 3β（p-GSK-3β），Cyclin D1，and fibrosis-related proteins，including transforming growth factor β1（TGF-β1），α-smooth muscle actin（α-SMA）， type I collagen（ColⅠ）and type Ⅲ collagen（Col Ⅲ）. Results Compared with the sham operation group，the endometrial tissue of rats in the model group and the low-，medium- and high- dose groups of polydatin showed different degrees of pathological damage，the thickness of the endometrium and the number of glands decreased， and there were obvious different degrees of collagen fiber deposition. The levels of TNF- α，IL-1β，IL-6，the relative protein expressions of β -catenin， Cyclin D1 and the activation level of GSK-3β protein， the relative protein expressions of TGF- β1， α -SMA， Col Ⅰ and Col Ⅲ in endometrial tissue were increased（P＜0.05）. Compared with the model group，the pathological damage of endometrial tissue in the low-，medium- and highdose groups of polydatin was improved，the endometrial thickness and the number of glands were increased，and the endometrial fibrosis was alleviated. In addition，the levels of the above indicators in the endometrial tissue were decreased（P＜0.05）in a dose-dependent manner. Conclusion Polydatin can promote endometrial repair and inhibit excessive endometrial fibrosis in rats with IUA，and its mechanism may be related to the inhibition of Wnt/ β-catenin signaling pathway activation.

R285.5

海南省自然科学基金资助项目（编号：822RC859）；海南省卫生计生行业科研项目（编号：20A200293）；海南省临床医学中心 建设项目资助（编号：琼卫医函 〔2021〕 75号）