【目的】探究芪丹颗粒抗脂肪细胞胰岛素抵抗 （IR） 机制。 【方法】采用棕榈酸培养法诱导3T3-L1脂肪细胞IR模型，设空 白对照组，模型组，芪丹颗粒低、中、高剂量组，芪丹颗粒+C1q/TNF相关蛋白3 （CTRP3） siRNA组。测定细胞上清中葡萄糖 消耗量；采用酶联免疫吸附分析 （ELISA） 法测定细胞上清中肿瘤坏死因子α （TNF-α） 和白细胞介素6 （IL-6） 含量；实时定量聚 合酶链反应 （qRT-PCR） 法检测细胞CTRP3、TNF-α、IL-6、葡萄糖转运蛋白4 （GLUT4） 基因表达；Western Blot法检测细胞中 CTRP3、磷脂酰肌醇-3激酶 （PI3K） 、蛋白激酶B （AKT） 的蛋白表达及其磷酸化水平。 【结果】与模型组比较，芪丹颗粒中、高 剂量组的葡萄糖消耗量升高并趋于空白对照组的70% ~ 82%，TNF-α含量降低了25% ~ 45%，IL-6含量下降了40% ~ 55%， GLUT4 mRNA表达水平增加了50%，CTRP3 mRNA及蛋白表达水平增加了25% ~ 40% （均P＜0.05） ；与芪丹颗粒组比较，芪 丹颗粒+CTRP3 siRNA组的葡萄糖消耗量和GLUT4 mRNA表达水平分别降低了33%和27%，IL-6和TNF-α的含量分别增加了 42%和41%，p-PI3K和p-AKT的蛋白表达水平增加了190%和180% （均P＜0.05） 。 【结论】芪丹颗粒可能通过促进CTRP3表 达降低炎症反应和激活PI3K/AKT信号通路来提高IR 3T3-L1脂肪细胞的胰岛素敏感性，改善脂肪细胞IR。

Objective To explore the mechanism of Qidan Granules prevent insulin resistance （IR） in adipocytes. Methods The IR model of 3T3-L1 adipocytes was induced by palmitic acid culture method. The blank control group，model group，Qidan Granules low-，medium- and high- dose groups，Qidan Granules + C1q/TNF- related protein 3 （CTRP3） siRNA group were set up. Determination of glucose consumption in cell supernatant was performed；the levels of tumor necrosis factor α （TNF-α） and interleukin 6 （IL-6） in the cell supernatant were determined by enzyme-linked immunosorbent assay （ELISA） . Real-time quantitative polymerase chain reaction （qRT-PCR） was used to detect the gene expressions of CTRP3， TNF- α， IL-6 and glucose transporter 4 （GLUT4） . The protein expressions and phosphorylation levels of CTRP3，phosphoinositide-3 kinase （PI3K） and protein kinase B （AKT） were detected by Western Blot. Results Compared with the model group，the glucose consumption was increased and tended to 70%-82% of the blank control group，TNF-α content was decreased by 25%-45%，IL-6 content was decreased by 40%-55%，GLUT4 mRNA expression level was increased by 50%， and mRNA and protein expression levels of CTRP3 were increased by 25%-40% in the medium- and high- dose groups of Qidan Granules （all P＜0.05） . Compared with the Qidan Granules group，the glucose consumption and GLUT4 mRNA expression level in the Qidan Granules + CTRP3 siRNA group were decreased by 33% and 27%， respectively，the contents of IL-6 and TNF-α were increased by 42% and 41%，respectively，and the protein expressions of p-PI3K and p-AKT were increased by 190% and 180%，respectively （all P＜0.05） . Conclusion Qidan Granules may improve the insulin sensitivity of IR 3T3-L1 adipocytes and improve IR of adipocytes by promoting CTRP3 expression to reduce inflammatory response and activate PI3K/AKT signaling pathway.

R285.5

深圳市科技计划项目 （编号：JCYJ20190807110803624）；深圳市宝安区科技计划项目 （编号：2021JD276）