探讨接骨七厘胶囊通过激活骨形态发生蛋白（BMP）/Smad信号通路对大鼠桡骨骨折愈合的改善作用。【方法】 （1）构建大鼠桡骨骨折模型，检测大鼠血清中碱性磷酸酶（ALP）、钙（Ca）和磷的含量，观察骨折间隙的病理学变化。（2）培养 人骨肉瘤细胞SaOS-2，检测ALP活性、矿化水平， 实时定量聚合酶链反应（qRT-PCR）法检测细胞成骨相关基因ALP、胶原 Ⅰ（COL-Ⅰ）、鸟氨酸转氨酶（OTC）、Osterix、骨桥蛋白（OPN）、Runt相关转录因子2（RUNX2）、BMP2表达，Western Blot法 检测细胞BMP/Smad信号通路中关键蛋白的表达。【结果】接骨七厘胶囊促进大鼠桡骨骨折愈合，增强ALP活性，增加钙和磷 含量。接骨七厘胶囊刺激SaOS-2细胞矿化结节的形成，并以剂量依赖的方式促进SaOS-2细胞中COL-I、OTC、Osterix、 BMP2 和OPN 的表达水平。接骨七厘胶囊处理可上调SaOS-2 细胞BMP/Smad 信号通路Smad1/5 磷酸化水平以及BMP2 和 RUNX2的水平。BMP/Smad信号通路的抑制剂Noggin抑制接骨七厘胶囊诱导的SaOS-2细胞成骨分化。【结论】接骨七厘胶囊 可通过激活BMP/Smad信号通路，提高成骨相关基因的表达，从而促进骨折愈合。

To investigate the improvement effect of Jiegu Qili Capsules on the fracture healing of radius in rats by activating the bone morphogenetic protein（BMP）/Smad signaling pathway. Methods（1）A rat model of radius fracture was constructed， and the serum levels of alkaline phosphatase （ALP）， calcium（Ca） and phosphorus were detected， and the pathological changes in the fracture gap was observed. （2）Human osteosarcoma SaOS-2 cells were used to measure ALP activity and mineralization level. The quantitative real-time polymerase chain reactionn（qRT-PCR）method was used to detect the cellular osteogenesis-related genes ALP， collagen I （COL- I）， ornithine transcarbamylase （OTC）， Osterix， osteoblastin （OPN）， Runt- related transcription factor 2（RUNX2）and BMP2. The expression of key proteins in BMP/Smad signal pathway was detected by Western Blot. Results Jiegu Qili Capsules effectively promoted fracture healing of radius in rats， enhance ALP activity，increase Ca and P levels Jiegu Qili Capsules stimulate the formation of mineralized nodules in SaOS-2 cells in rats.，and promoted the expression levels of COL-I，OTC，Osterix，BMP2 and OPN in SaOS- 2 cells in a dose-dependent manner. Jiegu Qili Capsules up-regulated the levels of Smad1/5 phosphorylation of the BMP/Smad signaling pathway in SaOS-2 cells，as well as the levels of BMP2 and RUNX2. Noggin，an inhibitor of the BMP/Smad signaling pathway， inhibited osteogenic differentiation of SaOS- 2 cells induced by Jiegu Qili Capsules. Conclusion Jiegu Qili Capsules can promote fracture healing by activating the BMP/Smad signaling pathway to increase the expression of osteogenesis-related genes

R285.5

2022年度长沙市自然科学基金项目（编号：kq2208197）